Review




Structured Review

STEMCELL Technologies Inc easyseptm beads (human t cell isolation kit)
Easyseptm Beads (Human T Cell Isolation Kit), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easyseptm+beads+(human+t+cell+isolation+kit)/easyseptm+beads++human+t+cell+isolation+kit+/pmc10424000-246-8-13
Average 90 stars, based on 1 article reviews
easyseptm beads (human t cell isolation kit) - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Selection:

Article Title: Methods of treating CART-T cell therapy-induced neuroinflammation using a GM-CSF antagonist
Article Snippet: Primary Cells and CAR-T Cells Peripheral blood mononuclear cells (PBMC) were isolated from de-identified normal donor blood apheresis cones obtained under a Mayo Clinic IRB approved protocol, using SepMate tubes (STEMCELL Technologies, Vancouver, Canada). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). .. Monocytes were isolated using a Human Monocyte Isolation Kit from Miltenyi Biotec, Bergisch Gladbach, Germany, which isolates CD14+ monocytes.

Article Title: Methods and materials for treating cancer
Article Snippet: Ex Vivo Generated CART Cells Peripheral blood mononuclear cells (PBMC) were isolated from de-identified donor blood apheresis cones using SepMate tubes (STEMCELL Technologies, Vancouver, Canada). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada), and monocytes were positively selected using CD14+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Primary cells were cultured in T Cell Media made with X-Vivo 15 (Lonza, Walkersville, MD, USA) with 10% human serum albumin (Corning Inc., Corning, NY, USA) and 1% Penicillin-Streptomycin-Glutamine (Gibco, Gaithersburg, MD, USA).

Article Title: CD19 occupancy with tafasitamab increases therapeutic index of CART19 cell therapy and diminishes severity of CRS
Article Snippet: 15 Peripheral blood samples from de-identified normal donor blood apheresis cones1 were 16 obtained under a Mayo Clinic Institutional Review Board (IRB) approved protocol and 17 PBMCs were isolated using SepMate tubes (STEMCELL Technologies, Vancouver, 18 Canada). .. T cells or NK cells were separated with negative selection magnetic beads 19 using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, 20 Canada) or EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies, 21 Vancouver, Canada), respectively according to the manufacturer’s instructions. ..

Article Title: Mesenchymal stromal cells with chimaeric antigen receptors for enhanced immunosuppression
Article Snippet: PBMCs were isolated from de-identified normal donor blood apheresis cones obtained under a Mayo Clinic Institutional Research Board approved protocol, using SepMate tubes (STEMCELL Technologies). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell 80 Isolation Kit (STEMCELL Technologies). .. Primary T cells and PBMCs were cultured in T-cell medium containing X-VIVO 15 (Lonza), 10% (v/v) human serum albumin (Innovative Research) and 1% (v/v) PSG (Gibco) before selection for in vitro co-culture.

Magnetic Beads:

Article Title: Methods of treating CART-T cell therapy-induced neuroinflammation using a GM-CSF antagonist
Article Snippet: Primary Cells and CAR-T Cells Peripheral blood mononuclear cells (PBMC) were isolated from de-identified normal donor blood apheresis cones obtained under a Mayo Clinic IRB approved protocol, using SepMate tubes (STEMCELL Technologies, Vancouver, Canada). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). .. Monocytes were isolated using a Human Monocyte Isolation Kit from Miltenyi Biotec, Bergisch Gladbach, Germany, which isolates CD14+ monocytes.

Article Title: Methods and materials for treating cancer
Article Snippet: Ex Vivo Generated CART Cells Peripheral blood mononuclear cells (PBMC) were isolated from de-identified donor blood apheresis cones using SepMate tubes (STEMCELL Technologies, Vancouver, Canada). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada), and monocytes were positively selected using CD14+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Primary cells were cultured in T Cell Media made with X-Vivo 15 (Lonza, Walkersville, MD, USA) with 10% human serum albumin (Corning Inc., Corning, NY, USA) and 1% Penicillin-Streptomycin-Glutamine (Gibco, Gaithersburg, MD, USA).

Article Title: CD19 occupancy with tafasitamab increases therapeutic index of CART19 cell therapy and diminishes severity of CRS
Article Snippet: 15 Peripheral blood samples from de-identified normal donor blood apheresis cones1 were 16 obtained under a Mayo Clinic Institutional Review Board (IRB) approved protocol and 17 PBMCs were isolated using SepMate tubes (STEMCELL Technologies, Vancouver, 18 Canada). .. T cells or NK cells were separated with negative selection magnetic beads 19 using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, 20 Canada) or EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies, 21 Vancouver, Canada), respectively according to the manufacturer’s instructions. ..

Article Title: Mesenchymal stromal cells with chimaeric antigen receptors for enhanced immunosuppression
Article Snippet: PBMCs were isolated from de-identified normal donor blood apheresis cones obtained under a Mayo Clinic Institutional Research Board approved protocol, using SepMate tubes (STEMCELL Technologies). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell 80 Isolation Kit (STEMCELL Technologies). .. Primary T cells and PBMCs were cultured in T-cell medium containing X-VIVO 15 (Lonza), 10% (v/v) human serum albumin (Innovative Research) and 1% (v/v) PSG (Gibco) before selection for in vitro co-culture.

Cell Isolation:

Article Title: Methods of treating CART-T cell therapy-induced neuroinflammation using a GM-CSF antagonist
Article Snippet: Primary Cells and CAR-T Cells Peripheral blood mononuclear cells (PBMC) were isolated from de-identified normal donor blood apheresis cones obtained under a Mayo Clinic IRB approved protocol, using SepMate tubes (STEMCELL Technologies, Vancouver, Canada). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). .. Monocytes were isolated using a Human Monocyte Isolation Kit from Miltenyi Biotec, Bergisch Gladbach, Germany, which isolates CD14+ monocytes.

Article Title: Methods and materials for treating cancer
Article Snippet: Ex Vivo Generated CART Cells Peripheral blood mononuclear cells (PBMC) were isolated from de-identified donor blood apheresis cones using SepMate tubes (STEMCELL Technologies, Vancouver, Canada). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada), and monocytes were positively selected using CD14+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Primary cells were cultured in T Cell Media made with X-Vivo 15 (Lonza, Walkersville, MD, USA) with 10% human serum albumin (Corning Inc., Corning, NY, USA) and 1% Penicillin-Streptomycin-Glutamine (Gibco, Gaithersburg, MD, USA).

Article Title: Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, Il, USA). .. T cells were negatively purified with EasySepTM beads (human T cell isolation kit, STEMCELL Technologies, Vancouver, Canada). .. T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%), Glutamax, and Penicillin/Streptomycin (100 U/mL/100 μg/mL) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and stimulated with 80 U/mL IL-2 (Peprotech, Rocky Hill, NJ, USA).

Article Title: CD19 occupancy with tafasitamab increases therapeutic index of CART19 cell therapy and diminishes severity of CRS
Article Snippet: 15 Peripheral blood samples from de-identified normal donor blood apheresis cones1 were 16 obtained under a Mayo Clinic Institutional Review Board (IRB) approved protocol and 17 PBMCs were isolated using SepMate tubes (STEMCELL Technologies, Vancouver, 18 Canada). .. T cells or NK cells were separated with negative selection magnetic beads 19 using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, 20 Canada) or EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies, 21 Vancouver, Canada), respectively according to the manufacturer’s instructions. ..

Article Title: Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments
Article Snippet: NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) , New England Biolabs , E7335S. .. EasySepTM Human T Cell Isolation Kit , Stemcell Technologies , 17954. .. RosetteSepTM Human Monocyte Enrichment Cocktail , Stemcell Technologies , 15064.

Purification:

Article Title: Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, Il, USA). .. T cells were negatively purified with EasySepTM beads (human T cell isolation kit, STEMCELL Technologies, Vancouver, Canada). .. T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%), Glutamax, and Penicillin/Streptomycin (100 U/mL/100 μg/mL) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and stimulated with 80 U/mL IL-2 (Peprotech, Rocky Hill, NJ, USA).

other:

Article Title: Qualitative differences in T‐cell activation by dendritic cell‐derived extracellular vesicle subtypes
Article Snippet: Total CD8 + T cells were isolated from CD14 negative fraction by negative selection using magnetic beads (EasySepTM Human CD8 + T Cell Isolation Kit, Stem Cell Technologies) following the manufacturer indications.

Article Title: METTL3 drives NAFLD-related hepatocellular carcinoma and is a therapeutic target for boosting immunotherapy
Article Snippet: EasySepTM Human T Cell Isolation Kit , STEMCELL , Cat# 17951.

Isolation:

Article Title: Mesenchymal stromal cells with chimaeric antigen receptors for enhanced immunosuppression
Article Snippet: PBMCs were isolated from de-identified normal donor blood apheresis cones obtained under a Mayo Clinic Institutional Research Board approved protocol, using SepMate tubes (STEMCELL Technologies). .. T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell 80 Isolation Kit (STEMCELL Technologies). .. Primary T cells and PBMCs were cultured in T-cell medium containing X-VIVO 15 (Lonza), 10% (v/v) human serum albumin (Innovative Research) and 1% (v/v) PSG (Gibco) before selection for in vitro co-culture.



Similar Products

90
STEMCELL Technologies Inc easyseptm beads (human t cell isolation kit)
Easyseptm Beads (Human T Cell Isolation Kit), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easyseptm+beads+(human+t+cell+isolation+kit)/easyseptm+beads++human+t+cell+isolation+kit+/pmc10424000-246-8-13
Average 90 stars, based on 1 article reviews
easyseptm beads (human t cell isolation kit) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results